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Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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JoVE Journal Neurociência
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

DOI:
10.3791/51351-v

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07:32 min

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June 23, 2014

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, , , , ,
1Department of Neurology-Neurosurgery,McGill University, 2Department of Biochemistry, Maisonneuve-Rosemont Hospital Research Centre,University of Montréal, 3Department of Ophthalmology, Maisonneuve-Rosemont Hospital Research Centre,University of Montréal

Capítulos

  • 00:05Título
  • 01:52Oxygen Induced Retinopathy (OIR) and Intravitreal Injection
  • 02:51Assessment of Vessel Perfusion and Barrier Function by Fluorescein Angiography
  • 03:29Enucleation and Eye Fixation
  • 04:16Retinal Dissection
  • 05:11Retinal Vascular Staining
  • 06:13Results: Vascular Regeneration in the Mouse Retina
  • 06:46Conclusion

Summary

Tadução automática

Tadução automática

The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.

Tags

Keywords: Vascular RegenerationCentral Nervous SystemMouse RetinaNeurovascular InterplayNeurodegenerative DiseasesRetinopathyDiabetic RetinopathyIschemic InjuryTherapeutic Modalities

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