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Myospheres的产生从人类胚胎干细胞通过表观遗传修饰
JoVE Journal
Biologia
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JoVE Journal Biologia
Generation of Myospheres From hESCs by Epigenetic Reprogramming
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Myospheres的产生从人类胚胎干细胞通过表观遗传修饰

DOI:
10.3791/51243-v

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09:32 min

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June 21, 2014

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1Muscle Development and Regeneration Program,Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

Capítulos

  • 00:05Título
  • 01:23Infection Procedure
  • 03:46Culturing the Infected Cells
  • 05:35EB-like Induction and Differentiation
  • 07:33Representative Results: Myogenic Differentiation of Human Embryonic Stem Cells
  • 08:56Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了基于人类胚胎干细胞（胚胎干细胞）的产生向着那宽容的培养条件下形成的收缩肌纤维（myospheres）三维簇骨骼肌祖细胞，它概括人类的生物学特性均匀人口表观遗传重编程的协议骨骼肌。

Tags

Keywords: HESCsSkeletal Muscle CellsMyospheresEpigenetic ReprogrammingMyoDBAF60CNeuromuscular Disorders3D Contractile StructuresStem Cell DifferentiationMiniaturized Skeletal Muscles

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