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评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
DOI:

08:11 min

September 01, 2015

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Capítulos

  • 00:05Título
  • 01:24Preparation of (Primary or MT4 CD4+) Target T Cells and (PBMC or pDC) Sensing Cells
  • 02:56Infected CD4+ T Cell Co-culture with Sensing Cells
  • 04:02Measurement of Bioactive Type-I IFN Using HEK-Blue IFN-α/β Reporter Cells
  • 05:23Results: Characterization and Co-culture of Freshly Isolated PBMCs and Infected MT4 T Cells
  • 07:27Conclusion

Summary

Tadução automática

不像无细胞的HIV-1颗粒,感染的CD4 + T细胞是由浆细胞样树突细胞(pDC细胞)有效地感测。这个手稿描述了一种方法,其中外周血单核细胞(PBMC)或分离的pDC是共培养的HIV-1感染的T细胞的pDC评估先天感测的评估,I型干扰素的释放。

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