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Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
JoVE Journal
Medicina
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JoVE Journal Medicina
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

DOI:
10.3791/51171-v

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07:13 min

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March 26, 2014

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, , , , , , , , , ,
1Tumor Microenvironment and Metastasis Program,Wistar Institute, 2Department of Pathology and Lab Medicine, Perelman School of Medicine,University of Pennsylvania, 3Department of Microbiology and Immunology and Department of Genetics,Geisel School of Medicine at Dartmouth, 4Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine,University of Pennsylvania, 5Rena Rowan Breast Center, Abramson Cancer Center,University of Pennsylvania, 6Center for Advanced Medicine,University of Pennsylvania

Capítulos

  • 00:05Título
  • 01:13Intraductal Injection of Virus Particles
  • 04:00Results: Representative Tumor Formation and Metastasis
  • 06:35Conclusion

Summary

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Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Tags

Keywords: Metastatic Breast CarcinomaTransgenic Mouse ModelDuctal EpitheliumAdenovirus-creP53 AblationK-ras OncogeneTumor ProgressionLymphovascular InvasionLeukocyte InfiltrationLatent MetastasisDormancyImmunotherapy

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