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Differential Markierung von Zelloberflächen und internalisierten Proteine Antikörper nach Fütterung der Live-kultivierten Neuronen

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

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Differential Markierung von Zelloberflächen und internalisierten Proteine Antikörper nach Fütterung der Live-kultivierten Neuronen

15,211 Views

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11:56 min

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February 12, 2014

DOI:

10.3791/51139-v

DOI:

10.3791/51139-v

•

11:56 min

February 12, 2014

•

5 Views

Nissa L. Carrodus*¹, Kathleen Sue-Lyn Teng*¹, Kathryn M. Munro, Matthew J. Kennedy, Jenny M. Gunnersen³

¹Department of Anatomy & Neuroscience,The University of Melbourne, ²Department of Neurobiology,Duke University Medical Center, ³Florey Institute of Neuroscience & Mental Health,The University of Melbourne

Tags

Differential Labeling Cell-surface Proteins Internalized Proteins Antibody Feeding Live Cultured Neurons Transmembrane Receptor Protein Trafficking Endocytosis Fluorescent Secondary Antibody Subcellular Localization

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