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细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记
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Neurociência
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JoVE Journal Neurociência
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记

15,210 Views

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11:56 min

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February 12, 2014

DOI:

10.3791/51139-v

DOI:
10.3791/51139-v

•

11:56 min
February 12, 2014

•

5 Views
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1Department of Anatomy & Neuroscience,The University of Melbourne, 2Department of Neurobiology,Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health,The University of Melbourne

Tags

Differential LabelingCell-surface ProteinsInternalized ProteinsAntibody FeedingLive Cultured NeuronsTransmembrane ReceptorProtein TraffickingEndocytosisFluorescent Secondary AntibodySubcellular Localization

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Carrodus, N. L., Teng, K. S., Munro, K. M., Kennedy, M. J., Gunnersen, J. M. Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons. J. Vis. Exp. (84), e51139, doi:10.3791/51139 (2014).

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