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检测持久性病毒的DNA在神经组织​​中的荧光的基因组与成绩单原位杂交结合免疫组化染色
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Neurociência
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JoVE Journal Neurociência
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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检测持久性病毒的DNA在神经组织​​中的荧光的基因组与成绩单原位杂交结合免疫组化染色

DOI:
10.3791/51091-v

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13:22 min

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January 23, 2014

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, , ,
1Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire,CNRS UMR 5534, 2Université de Lyon 1, 3Laboratoire d’excellence,LabEX DEVweCAN, 4Institut de Virologie Moléculaire et Structurale,CNRS UPR 3296, 5Centre de Recherche en Cancérologie de Lyon, INSERM U1052,CNRS UMR 5286

Capítulos

  • 00:05Título
  • 02:27Animal Infection
  • 03:20Trigeminal Ganglia Harvesting and Sectioning
  • 05:02DNA-FISH
  • 10:08Protocol Development and Applications
  • 12:32Conclusion

Summary

Tadução automática

Tadução automática

我们原位杂交协议建立一个荧光供于动物模型的组织切片中的持久DNA病毒基因组的检测。这个协议允许研究感染过程由codetection病毒基因组，它的RNA产物，以及单细胞内的病毒或细胞蛋白。

Tags

Keywords: DNA-FISHRNA-FISHImmunofluorescenceHSV-1LatencyGene Expression RegulationSingle Cell Analysis

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