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Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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JoVE Journal Química
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

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13:59 min

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December 12, 2013

DOI:

10.3791/50875-v

DOI:
10.3791/50875-v

•

13:59 min
December 12, 2013

•

3 Views
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1Department of Chemistry,Louisiana State University

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NMRNuclear Magnetic ResonanceReductive 13C-methylation13C-dimethylamineProtein StructureProtein DynamicsMass SpectrometryLysozymeN-terminal AmineLysine Side Chain

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Roberson, K. J., Brady, P. N., Sweeney, M. M., Macnaughtan, M. A. Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins. J. Vis. Exp. (82), e50875, doi:10.3791/50875 (2013).

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