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La base di Cell-L-Glutatione Protezione saggi per studiare endocitosi e riciclo dei Plasma proteine ​​di membrana
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
DOI:

09:22 min

December 13, 2013

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Capítulos

  • 00:05Título
  • 01:45Endocytic Assay: Biotinylation of the Apical Membrane Domain
  • 07:54Results: CFTR Endocytosis in CFBE41o- Cells
  • 08:49Conclusion

Summary

Tadução automática

Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

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