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Calcium Phosphate Transfection of Primary Hippocampal Neurons
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Neurociência
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JoVE Journal Neurociência
Calcium Phosphate Transfection of Primary Hippocampal Neurons

Calcium Phosphate Transfection of Primary Hippocampal Neurons

DOI:
10.3791/50808-v

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08:22 min

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November 12, 2013

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, , , ,
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School,Rutgers University

Capítulos

  • 00:05Título
  • 01:06Preparing Rat Astrocyte Cultures
  • 04:08Neuronal Culture
  • 05:39Calcium Phosphate Transfection
  • 07:23Transfection Efficiency and Neuronal Health
  • 08:02Conclusion

Summary

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Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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Calcium PhosphateTransfectionPrimary Hippocampal NeuronsAstroglial CellsCultureProtocol

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