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Protokolle für die Implementierung einer<em> Escherichia coli</em> Basierend TX-TL Zellfreie Expression System für Synthetische Biologie

JoVE Journal
Biologia

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JoVE Journal Biologia

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

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Protokolle für die Implementierung einer<em> Escherichia coli</em> Basierend TX-TL Zellfreie Expression System für Synthetische Biologie

60,980 Views

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16:11 min

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September 16, 2013

DOI:

10.3791/50762-v

DOI:

10.3791/50762-v

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16:11 min

September 16, 2013

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14 Views

Zachary Z. Sun*¹, Clarmyra A. Hayes*², Jonghyeon Shin, Filippo Caschera, Richard M. Murray, Vincent Noireaux

¹Department of Biology,California Institute of Technology, ²Department of Bioengineering,California Institute of Technology, ³Synthetic Biology Center, Department of Bioengineering,Massachusetts Institute of Technology, ⁴School of Physics and Astronomy,University of Minnesota

Tags

Cell-free Expression System Escherichia Coli TX-TL Transcription-translation Synthetic Biology Protein Expression In Vitro Endogenous T7 RNA Polymerase Lac/tet Repressors MRNA Degradation DNA Degradation Biomolecular Breadboard

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