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Protocolli per l'implementazione di un<em> Escherichia coli</em> Sulla base TX-TL cellulare-Free Expression System per la biologia sintetica
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Biologia
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JoVE Journal Biologia
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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Protocolli per l'implementazione di un<em> Escherichia coli</em> Sulla base TX-TL cellulare-Free Expression System per la biologia sintetica

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16:11 min

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September 16, 2013

DOI:

10.3791/50762-v

DOI:
10.3791/50762-v

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16:11 min
September 16, 2013

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82 Views
, , , , ,
1Department of Biology,California Institute of Technology, 2Department of Bioengineering,California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering,Massachusetts Institute of Technology, 4School of Physics and Astronomy,University of Minnesota

Summary

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Questo protocollo di cinque giorni delinea tutte le fasi, attrezzature e software supplementare necessario per la creazione e l'esecuzione di un sistema di espressione TX-TL cell-free da zero efficiente Escherichia coli endogeno basato. Con i reagenti, il protocollo dura 8 ore o meno per impostare una reazione, raccogliere e elaborare i dati.

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Cite this Article

Sun, Z. Z., Hayes, C. A., Shin, J., Caschera, F., Murray, R. M., Noireaux, V. Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology. J. Vis. Exp. (79), e50762, doi:10.3791/50762 (2013).

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