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在纯化造血干/祖细胞识别DNA突变
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
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在纯化造血干/祖细胞识别DNA突变

12,849 Views

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11:06 min

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February 24, 2014

DOI:

10.3791/50752-v

DOI:
10.3791/50752-v

•

11:06 min
February 24, 2014

•

3 Views
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1Greehey Children’s Cancer Research Institute,UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology,UT Health Science Center at San Antonio, 3Department of Pathology,UT Health Science Center at San Antonio, 4Department of Microbiology,UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center,UT Health Science Center at San Antonio

Summary

Automatically generated

这里我们描述了对小数目使用了LacI转基因小鼠模型纯化造血干细胞的体内诱变法。该LacI的基因可以分离来确定频率，位置和类型的DNA突变的自发产生或暴露于基因毒素后。

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Cite this Article

Cheng, Z., Zhou, T., Merchant, A., Prihoda, T. J., Wickes, B. L., Xu, G., Walter, C. A., Rebel, V. I. Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells. J. Vis. Exp. (84), e50752, doi:10.3791/50752 (2014).

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