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神经元的分化的人类Neuroprogenitor细胞培养激光捕获显微切割

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

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神经元的分化的人类Neuroprogenitor细胞培养激光捕获显微切割

DOI:

10.3791/50487-v

•

12:38 min

•

September 16, 2013

•

Ron Bouchard², Thomas Chong², Subbiah Pugazhenthi²

¹Section of Endocrinology,Denver VA Medical Center, ²Department of Medicine,University of Colorado Denver School of Medicine

Capítulos

00:00Título
01:51Expansion and Differentiation of Human Neuroprogenitor Cells
05:28Immunofluorescence Staining of Differentiated Neuroprogenitor
10:17Results: Laser Capture Microdissection of Differrntiated Neurons
12:14Conclusion

Summary

Tadução automática

人类neuroprogenitor细胞（的NPC）增殖的条件下扩大。的NPC分化成神经营养因子中的组合的存在下神经元的富培养物。用免疫组化法检测神经元标志物。为了分离神经元的纯人口的NPC分化的PEN膜幻灯片并进行激光捕获显微切割。

Tags

Laser Capture Microdissection Neurons Differentiated Human Neuroprogenitor Cells Cell Culture Neuronal Markers Astrocyte Markers NeuN Synapsin Acetylcholinesterase Synaptophysin GAP43 GFAP STAT3 Molecular Characterization

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