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人类心肌细胞的生成：从馈线人类诱导多能干细胞的分化协议

JoVE Journal
Biologia

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JoVE Journal Biologia

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

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人类心肌细胞的生成：从馈线人类诱导多能干细胞的分化协议

DOI:

10.3791/50429-v

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13:18 min

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June 28, 2013

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Elisa Di Pasquale², Belle Song, Gianluigi Condorelli

¹Humanitas Clinical and Research Center, Italy, ²Institute of Genetic and Biomedical Research (IRGB),National Research Council (CNR)

Capítulos

00:05Título
02:15Feeder-free Maintenance and Passaging of Human iPS Cell Lines
03:54Embryoid Bodies Aggregation and Cardiac Induction
06:25Beating Areas Isolation and Culture
07:48Single Beating Cells Isolation and Analysis
10:06Characterizing Contracting Cardiomyocytes Derived from iPS Cells
12:36Conclusion

Summary

Tadução automática

多能干细胞，胚胎或诱导多能干细胞（iPS），构成人类分化的细胞，包括心肌细胞的重要来源。在这里，我们将专注于心脏iPS细胞的诱导，展示如何使用它们来获得功能的人类心肌细胞通过胚状体为基础的协议。

Tags

Keywords: Human Cardiomyocytes Induced Pluripotent Stem Cells Differentiation Protocol Feeder-free Heart Development Myocardial Diseases Drug Discovery Toxicology Cardiac Disorders Embryoid Bodies Chemically Defined Protocols

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