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从脐带血分离的前体B细胞亚群

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

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从脐带血分离的前体B细胞亚群

DOI:

10.3791/50402-v

•

14:06 min

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April 16, 2013

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Md Almamun, Jennifer L. Schnabel, Susan T. Gater, Jie Ning, Kristen H. Taylor

¹Department of Pathology and Anatomical Sciences,University of Missouri-Columbia, ²Laboratory for Infectious Disease Research,University of Missouri-Columbia

Capítulos

00:05Título
02:12Isolation of Mononuclear Cells from Umbilical Cord Blood
04:19Modified B-cell Isolation from Mononuclear Cells Using MACS Separation
06:32Antibody Labeling and Preparation for Cell Sorting
08:32Cell Sorting Using the MoFlo XDP Flow Cytometer
11:07Results: Isolation of Cord Blood Pre-cursor B-cell Subsets
13:13Conclusion

Summary

Tadução automática

在这里，我们描述的协议子集的前体B细胞从脐带血中隔离开来。阿足够的数量和质量，可从细胞中提取核酸，并且用于在随后的实验，利用DNA或RNA。

Tags

Umbilical Cord Blood Hematopoietic Progenitor Cells Precursor B-cells B-cell Differentiation Cell Isolation Cell Sorting Cell Surface Markers Mononuclear Cells Biotin Fluorescent Labeling Downstream Nucleic Acid Assays

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