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前子宫穿孔和整个半球的外植体早期皮质发育的研究：一个简单的实验方法

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

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前子宫穿孔和整个半球的外植体早期皮质发育的研究：一个简单的实验方法

DOI:

10.3791/50271-v

•

13:47 min

•

April 03, 2013

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Anna J. Nichols*¹, Ryan S. O’Dell*¹, Teresa A. Powrozek*¹, Eric C. Olson

¹Department of Neuroscience and Physiology,SUNY Upstate Medical University

Capítulos

00:05Título
02:00Ex utero Electroporations with GFP
04:04Whole Hemisphere Explant Preparation
06:25Fixation, Embedding, and Sectioning
09:30Neuronal Development in Injected, Cultured, and Processed Hemispheres
13:11Conclusion

Summary

Tadução automática

该协议介绍了一种改进的外植体的过程，涉及到<em>子宫外</em电，从小鼠胚胎的整个大脑半球的解剖和文化。编制有利于早期皮质发育过程中的基因功能的药理研究和分析。

Tags

Keywords: Cortical Development Cerebral Hemisphere Explant Ex Utero Electroporation Organotypic Environment Neuronal Migration Neuronal Differentiation Live Imaging Preplate Splitting Cortical Layer Formation

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