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同时前和突触后电生理记录<em>爪蟾</em神经肌肉文化
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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同时前和突触后电生理记录<em>爪蟾</em神经肌肉文化

DOI:
10.3791/50253-v

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08:13 min

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March 11, 2013

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, , ,
1Department of Physiology,David Geffen School of Medicine at UCLA, 2Natural Science Division,Pepperdine University

Capítulos

  • 00:05Título
  • 01:41Pre-experimental Preparation
  • 03:24Microdissection of Xenopus embryos
  • 04:51Preparation of Nerve-muscle Co-cultures
  • 05:52Patch Clamping Nerve-muscle Synapses
  • 07:02Results: Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

本视频演示了用来种植原代培养的胚胎的程序<em>爪蟾</em>神经和肌肉细胞和该制剂的同时前和突触后的膜片钳记录的用处。

Tags

Keywords: XenopusNerve-muscle Co-cultureElectrophysiologyPresynapticPostsynapticNeurotransmitter ReleaseIon ChannelsSynaptic InteractionsSynaptic DevelopmentSynaptic Plasticity

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