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1Department of Neurology, McKnight Brain Institute,University of Florida , 2Department of Entomology and Nematology,University of Florida , 3Genetics Institute, Department of Molecular Genetics and Microbiology,University of Florida , 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration,University of Florida
Capítulos
- 00:05Título
- 01:24Generating a Replicate and Freezing Flies
- 02:42Collection and Lyophilization of Fly Heads
- 03:53RNA-specific Procedures: Total RNA Extraction and mRNA Purification
- 07:23Metabolite-specific Procedures: Methanol-chloroform Extraction
- 09:54Representative Results
- 12:23Conclusion
Vi beskriver her procedurerne for ekstraktion og oprensning af mRNA og nedbrydningsprodukter fra<em> Drosophila</em> Hoveder. Vi anvender disse teknikker til bedre at forstå de cellulære perturbationer underliggende neuronal degeneration. Disse metoder kan let skaleres og tilpasses til andre "OMIC" projekter.
