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Neonatal Subventricular Zone Elektroporation
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Neurociência
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JoVE Journal Neurociência
Neonatal Subventricular Zone Electroporation

Neonatal Subventricular Zone Elektroporation

DOI:
10.3791/50197-v

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08:06 min

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February 11, 2013

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, ,
1Department of Neurosurgery and Cellular & Molecular Physiology,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 01:39Preparation of DNA, Solutions, and Glass Pipettes
  • 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
  • 05:04Electroporation and Recovery
  • 06:44Results: Images of an Electroporated Subventricular Zone
  • 07:48Conclusion

Summary

Tadução automática

Tadução automática

Vi viser en minimalt invasiv teknik kaldet neonatal subventricular zone elektroporering. Teknikken består i at injicere plasmid-DNA i de laterale ventrikler af neonatale hvalpe og anvendelse af elektrisk strøm til at levere og genmanipulere neurale stamceller

Tags

Keywords: Neural Stem CellsNeonatal Subventricular ZoneElectroporationGenetic ManipulationNeural Progenitor CellsCentral Nervous SystemCortical DevelopmentForebrain

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