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新生儿脑室下区电穿孔
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Neurociência
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JoVE Journal Neurociência
Neonatal Subventricular Zone Electroporation
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新生儿脑室下区电穿孔

DOI:
10.3791/50197-v

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08:06 min

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February 11, 2013

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, ,
1Department of Neurosurgery and Cellular & Molecular Physiology,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 01:39Preparation of DNA, Solutions, and Glass Pipettes
  • 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
  • 05:04Electroporation and Recovery
  • 06:44Results: Images of an Electroporated Subventricular Zone
  • 07:48Conclusion

Summary

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我们展示出一种微创技术，新生儿脑室下区电。该技术包括新生幼崽的侧脑室注射质粒DNA和通电时提供神经干细胞和基因操纵

Tags

Keywords: Neural Stem CellsNeonatal Subventricular ZoneElectroporationGenetic ManipulationNeural Progenitor CellsCentral Nervous SystemCortical DevelopmentForebrain

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