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Neonatal Subventricular Zone Electroporation
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Neurociência
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JoVE Journal Neurociência
Neonatal Subventricular Zone Electroporation

Neonatal Subventricular Zone Electroporation

DOI:
10.3791/50197-v

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08:06 min

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February 11, 2013

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, ,
1Department of Neurosurgery and Cellular & Molecular Physiology,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 01:39Preparation of DNA, Solutions, and Glass Pipettes
  • 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
  • 05:04Electroporation and Recovery
  • 06:44Results: Images of an Electroporated Subventricular Zone
  • 07:48Conclusion

Summary

Tadução automática

Tadução automática

We demonstrate a minimally invasive technique referred to as neonatal subventricular zone electroporation. The technique consists of injecting plasmid DNA into the lateral ventricles of neonatal pups and applying electrical current to deliver and genetically manipulate neural stem cells

Tags

Keywords: Neural Stem CellsNeonatal Subventricular ZoneElectroporationGenetic ManipulationNeural Progenitor CellsCentral Nervous SystemCortical DevelopmentForebrain

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