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Gecombineerd Immunofluorescentie en DNA FISH op 3D-bewaarde interfase kernen aan veranderingen in 3D Nuclear Organisatie Studie
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Biologia
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JoVE Journal Biologia
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
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Gecombineerd Immunofluorescentie en DNA FISH op 3D-bewaarde interfase kernen aan veranderingen in 3D Nuclear Organisatie Studie

17,957 Views

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13:55 min

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February 03, 2013

DOI:

10.3791/50087-v

DOI:
10.3791/50087-v

•

13:55 min
February 03, 2013

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2 Views
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1Department of Pathology,New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center,Yale University School of Medicine

Tags

3D DNA FISH3D Nuclear Organization3D Confocal MicroscopyImmunofluorescenceChromatin ModificationsGene LociChromosome TerritoriesNuclear ArchitectureNuclear ProteinsHistone VariantsTranscription MachineryNuclear Sub-compartments

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Chaumeil, J., Micsinai, M., Skok, J. A. Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization. J. Vis. Exp. (72), e50087, doi:10.3791/50087 (2013).

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