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临床应用<em>睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
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临床应用<em>睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中

DOI:
10.3791/50070-v

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09:29 min

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February 01, 2013

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, , , , , , , ,
1Division of Pediatrics,U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Capítulos

  • 00:05Título
  • 02:09Isolation of Mononuclear Cells from PB and UCB
  • 03:04Preparation of T Cells for Electroporation
  • 04:03Electroporation of MNC
  • 05:59FACS Analysis of CAR Expression (Day 1)
  • 06:32Preparation of aAPC (Clone #4) on Day 1
  • 07:01aAPC-mediated Stimulation of CAR+ T Cells (Day 1)
  • 07:33Results: SB System Coupled with Co-culture on aAPC Generates CD19-specific CAR+ T Cells
  • 08:44Conclusion

Summary

Tadução automática

Tadução automática

T细胞表达CD19-特异性嵌合抗原受体（CAR）被注入我们的第一个人类基因治疗试验中研究治疗B细胞恶性肿瘤。我们描述了使用基因改造的T细胞<em>睡美人</em（SB）系统地介绍具体的CD19-CAR和选择性传播的名牌CD19 +人工抗原提呈细胞。

Tags

Keywords: Sleeping BeautyArtificial Antigen Presenting CellsGenetic ModificationT CellsPeripheral BloodUmbilical Cord BloodCAR T CellsCD19Gene TherapyImmunotherapyTumor-associated AntigensClinical Application

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