/
/
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
12,991 Views
•
12:58 min
•
November 20, 2012
DOI:
Tags
PHluorin-tagged Receptor InsertionPlasma MembranePrimary Cultured Mouse NeuronsReceptor TraffickingSpatial ResolutionTemporal ResolutionVesicle Detection SensitivitySurface BiotinylationBiochemical DetectionFluorescence MicroscopyImmunolabeled Surface ReceptorsDynamic Insertion VisualizationSuperecliptic PHluorinPH-sensitive GFP
Read Article
Cite this Article
Li, Y., Roy, B. D., Wang, W., Zhang, L., Sampson, S. B., Lin, D. Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons. J. Vis. Exp. (69), e4450, doi:10.3791/4450 (2012).
Copy