/
/
Bildanalys av neuron till Glia interaktion i mikroflödessystem Kultur Platform (MCP)-baserade neuronal Axon och Glia samodlingssystemet
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:27Assembly of the Microfluidic Culture Chamber (MCP)
- 02:43Preparation of Neuronal Culture
- 03:33Induction of Neuronal Axons in Assembled MCP
- 04:48A Compartmentalized Co-culture System: Adding Cultured Astrocytes
- 06:15Results: Axon-induced GLT1 Promoter Activation in Astrocytes
- 09:07Conclusion
Denna studie beskriver förfaranden för att inrätta en ny neuronal axon och (astro) Glia samodling plattform. I denna samodlingssystemet blir manipulering av direkt interaktion mellan en axon (och enda gliaceller) genomförbart, så mekanistiska analys av ömsesidiga neuron till gliala signalering.
Tags
Imaging AnalysisNeuronGlia InteractionMicrofluidic Culture PlatformMCP-based Neuronal Axon And Glia Co-culture SystemSignaling PathwaysCentral Nervous System (CNS)Neuron And Glia Mixed CulturesNeuronal CompartmentsAxonDendriteSomaCulture SystemSoluble FactorsDirect Membrane Contact SignalsSingle AxonGlial Cell
