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Mobiele Tracking Met behulp van Photoconvertible eiwitten tijdens ontwikkeling van de zebravis
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Biologia
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JoVE Journal Biologia
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
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Mobiele Tracking Met behulp van Photoconvertible eiwitten tijdens ontwikkeling van de zebravis

DOI:
10.3791/4350-v

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07:19 min

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September 28, 2012

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, ,
1Max Delbrück Center for Molecular Medicine

Capítulos

  • 00:05Título
  • 01:30Choose the Brightest Embryo and Embedding It for Photoconversion Assay
  • 02:52Photoconversion and Embryonic Development and Tracking Photoconverted Tissue
  • 05:11Representative Photoconversion Results
  • 06:56Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.

Tags

Cell TrackingPhotoconvertible ProteinsZebrafish DevelopmentEmbryogenesisGenetically-encoded Fluorescent ProteinsDynamic Morphogenetic ProcessesPhotoactivatable ProteinsPhotoconvertible Fluorescent Proteins (PCFPs)KaedeKikGRDendraEosFPGreen To Red ShiftUV Or Blue Light ExposureCell Fate Tracking

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