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Substrat Generation for endonukleaser af CRISPR / Cas Systems
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September 08, 2012
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Summary
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CRISPR / Cas-systemer medierer adaptive immunitet i Bacteria og Archaea. Mange Cas proteiner foreslået at fungere som endoribonucleases virker på crRNA forstadier af varierende længde. Her viser vi tre forskellige tilgange til at generere pre-crRNA substrater for den biokemiske analyse af Cas endonuclease aktivitet.
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Substrate GenerationEndonucleasesCRISPR/Cas SystemsVirusesProkaryotic HostsBacterial EvolutionArchaeal LifeRestriction ModificationAbortive InfectionAdaptive Immune SystemsCRISPR SequencesCas GenesCas ProteinsSpacer ElementCRISPR ClusterPrecursor-crRNACas6 FamilyCrRNAsViral DNA UptakeCascade ComplexBase ComplementarityNuclease Cas3Invader DNA Destruction
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Zoephel, J., Dwarakanath, S., Richter, H., Plagens, A., Randau, L. Substrate Generation for Endonucleases of CRISPR/Cas Systems. J. Vis. Exp. (67), e4277, doi:10.3791/4277 (2012).
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