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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Biologia
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JoVE Journal Biologia
Substrate Generation for Endonucleases of CRISPR/Cas Systems

Substrate Generation for Endonucleases of CRISPR/Cas Systems

26,890 Views

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11:53 min

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September 08, 2012

DOI:

10.3791/4277-v

DOI:
10.3791/4277-v

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11:53 min
September 08, 2012

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1Prokaryotic Small RNA Biology,Max-Planck-Institute for Terrestrial Microbiology

Tags

Substrate GenerationEndonucleasesCRISPR/Cas SystemsVirusesProkaryotic HostsBacterial EvolutionArchaeal LifeRestriction ModificationAbortive InfectionAdaptive Immune SystemsCRISPR SequencesCas GenesCas ProteinsSpacer ElementCRISPR ClusterPrecursor-crRNACas6 FamilyCrRNAsViral DNA UptakeCascade ComplexBase ComplementarityNuclease Cas3Invader DNA Destruction

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Zoephel, J., Dwarakanath, S., Richter, H., Plagens, A., Randau, L. Substrate Generation for Endonucleases of CRISPR/Cas Systems. J. Vis. Exp. (67), e4277, doi:10.3791/4277 (2012).

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