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En parasit Rescue och Transformation analys för Antileishmanial screening mot intracellulära<em> Leishmania donovani</em> Amastigotes i THP1 Human akut monocytisk leukemicellinjen
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Capítulos
- 00:05Título
- 02:44Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide
- 04:13Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes
- 05:43Treatment of Infected Macrophages with Test Drugs
- 06:43Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect
- 08:09Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages
- 08:59Quantitative Analysis of Transformed Promastigotes
- 09:22Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug
- 10:41Conclusion
En parasit-räddning och omvandling analys med THP1 infekterade celler<em> In vitro</em> Med<em> Leishmania donovani</em> Har optimerats för anti-leishmanial drog screening. Analysen omfattar differentiering av THP1 celler, infektion med promastigotes, behandling med testläkemedel, kontrollerad lys av infekterade makrofager, räddning av amastigotes, omvandling till promastigotes och övervakning promastigote tillväxt och spridning med fluorometrisk analys.
Tags
Parasite RescueTransformation AssayAntileishmanial ScreeningIntracellular Leishmania Donovani AmastigotesTHP1 Human Acute Monocytic Leukemia Cell LineLeishmaniasisNeglected DiseasesDeveloping CountriesRisk Of Contracting LeishmaniasisVisceral LeishmaniasisLimited Drug OptionsTherapeutic Doses ToxicityMultiple Drug ResistanceAnti-leishmanial Drug Discovery PipelineIn Vitro Screening ModelsPromastigotes AssayAxenic Amastigotes AssayMacrophage-amastigotes Model
