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En Parasite Rescue and Transformation Assay for Antileishmanial Screening mot intracellulære<em> Leishmania donovani</em> Amastigotes i THP1 Menneskelig Akutt monocyttisk leukemi cellelinje
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Capítulos
- 00:05Título
- 02:44Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide
- 04:13Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes
- 05:43Treatment of Infected Macrophages with Test Drugs
- 06:43Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect
- 08:09Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages
- 08:59Quantitative Analysis of Transformed Promastigotes
- 09:22Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug
- 10:41Conclusion
En parasitt-redning og transformasjon analysen med THP1 celler infisert<em> In vitro</em> Med<em> Leishmania donovani</em> Har blitt optimalisert for anti-leishmanial narkotika screening. Analysen omfatter differensiering av THP1 celler, infeksjon med promastigotes, behandling med test narkotika, kontrollert lyse av de infiserte makrofager, redning av amastigotes, transformasjon til promastigotes og overvåking promastigote vekst og spredning med en fluorometrisk analyse.
Tags
Parasite RescueTransformation AssayAntileishmanial ScreeningIntracellular Leishmania Donovani AmastigotesTHP1 Human Acute Monocytic Leukemia Cell LineLeishmaniasisNeglected DiseasesDeveloping CountriesRisk Of Contracting LeishmaniasisVisceral LeishmaniasisLimited Drug OptionsTherapeutic Doses ToxicityMultiple Drug ResistanceAnti-leishmanial Drug Discovery PipelineIn Vitro Screening ModelsPromastigotes AssayAxenic Amastigotes AssayMacrophage-amastigotes Model
