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細胞内の抗リーシュマニアに対するスクリーニングのための寄生虫のRescue and形質転換試験<em>ドノバンリーシュマニア</emTHP1ヒト急性単球性白血病細胞株における>無鞭毛
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Capítulos
- 00:05Título
- 02:44Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide
- 04:13Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes
- 05:43Treatment of Infected Macrophages with Test Drugs
- 06:43Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect
- 08:09Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages
- 08:59Quantitative Analysis of Transformed Promastigotes
- 09:22Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug
- 10:41Conclusion
Tags
Parasite RescueTransformation AssayAntileishmanial ScreeningIntracellular Leishmania Donovani AmastigotesTHP1 Human Acute Monocytic Leukemia Cell LineLeishmaniasisNeglected DiseasesDeveloping CountriesRisk Of Contracting LeishmaniasisVisceral LeishmaniasisLimited Drug OptionsTherapeutic Doses ToxicityMultiple Drug ResistanceAnti-leishmanial Drug Discovery PipelineIn Vitro Screening ModelsPromastigotes AssayAxenic Amastigotes AssayMacrophage-amastigotes Model
