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为揭示复杂的大脑地形wholemount免疫组化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

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为揭示复杂的大脑地形wholemount免疫组化

DOI:

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09:23 min

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April 05, 2012

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Joshua J. White, Stacey L. Reeber, Richard Hawkes, Roy V. Sillitoe

¹Department of Neuroscience,Albert Einstein College of Medicine, Yeshiva University , ²Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine,University of Calgary

Capítulos

00:05Título
01:00Dissection of the Cerebellum
02:05Processing the Tissue for Wholemount Staining
06:10Imaging the Stained Tissue
06:49Results: Zebrin II and CART Immunohistochemistry in Wholemount Cerebella
08:55Conclusion

Summary

Tadução automática

神经回路地形组织功能的车厢与特定的分子型材。在这里，我们提供一个多功能wholemount免疫组织化学染色方法为揭示全球脑地形使用的实际和技术步骤。我们证明了实用的使用方法很好理解的小脑细胞构筑和电路。

Tags

Wholemount Immunohistochemistry Brain Topography Cerebellum Cellular Architecture Gene Expression Domains Protein Expression Domains Molecular Compartmentalization Afferent Fibers Patterning Defects Mouse Cerebellum Wholemount Staining Approach Protein Expression Patterns ZebrinII/aldolaseC Expression Three-dimensional Conformation Afferent Projections Comparative Studies Molecular Topography Rat Cerebellum

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