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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
 

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Article DOI: 10.3791/4040-v • 09:25 min • May 21st, 2012
May 21st, 2012 •
1, 1, 2, 1, 1,2
1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

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Resumo
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Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.

Tags

Isolation Characterization Dendritic Cells Macrophages Mouse Intestine Innate Immune Cells Adaptive Immune Cells Tolerance Commensal Flora Food Antigens Inflammatory Responses Antigen Presenting Cells DCs Macrophages Intestinal Immune Homeostasis Microbiota Isolation Methods Digestion Times Cell Surface Antigen Expression Cell Viability Cell Yield Phenotypic Characterization Fluorescence-labeled Monoclonal Antibodies Flow Cytometric Analysis Functional Studies CD11c CD11b Magnetic-activated Cell Sorting Beads
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