É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
12,505 Views
•
10:40 min
•
August 04, 2012
DOI:
Summary
Automatically generated
An optical system was developed to visualize hepatic microcirculation with FITC-labeled erythrocytes and to measure the partial pressure of oxygen in the microvessels with laser-assisted phosphorimetry. This method can be used to investigate physiological and pathological mechanisms by analyzing microvascular structure, diameter, blood flow velocity, and oxygen tension.
Explore More Videos
Blood FlowOxygen ConsumptionHepatic MicrocirculationAcute HepatitisOxygen SupplyOxygen DemandPortal Vein BloodGastrointestinal TractSpleenHepatocytesSinusoidsOxygen GradientEnzymesOxygen MetabolismFITC-labeled ErythrocytesLaser-assisted PhosphorimetryPartial Pressure Of OxygenBlood Flow VelocitiesVessel DiametersHepatocyte Necrosis
Read Article
Cite this Article
Tsukada, K., Suematsu, M. Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model. J. Vis. Exp. (66), e3996, doi:10.3791/3996 (2012).
Copy