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从使用吸吸液管技术的嗅觉受体神经元的气味引起的反应记录
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JoVE Journal Neurociência
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
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从使用吸吸液管技术的嗅觉受体神经元的气味引起的反应记录

DOI:
10.3791/3862-v

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08:08 min

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April 05, 2012

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, , ,
1Monell Chemical Senses Center, 2Department of Physiology, Development & Neuroscience, Physiological Laboratory,University of Cambridge

Capítulos

  • 00:05Título
  • 01:30Making Electrodes
  • 02:41Isolation of Olfactory Receptor Neurons
  • 04:18Recordings
  • 06:28Results: Odorant-induced Responses Recorded from ORNs using the Suction Pipette Technique
  • 07:46Conclusion

Summary

Tadução automática

Tadução automática

嗅觉受体神经元（ORNs）气味信号转换成一个受体的电流，从而触发动作电位，被输送到第二个神经元在嗅球中。这里，我们描述的吸入移液管的技术，同时记录从小鼠ORNs电流和动作电位的加臭剂诱导的受体。

Tags

Odorant-induced ResponsesOlfactory Receptor NeuronsSuction Pipette TechniqueChemosensory SystemsNasal CavityOlfactory EpitheliumSignal Transduction MachineryExcitatory Current InfluxCiliary Transduction ChannelsCyclic Nucleotide-gated ChannelCa2+-activated Cl- ChannelDepolarizationAction Potential GenerationSuction Pipette Technique For Recording ORN Responses

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