Locked Nucleic Acid Flow Cytometry-fluorescence <em>in situ</em> Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Kelly L. Robertson; Gary J. Vora

doi:10.3791/3655

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Flujo de ácido nucleico cerrado Citometría de fluorescencia In situ (LNA flujo-FISH): un método para la detección de bacterias pequeñas de ARN

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

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Flujo de ácido nucleico cerrado Citometría de fluorescencia In situ (LNA flujo-FISH): un método para la detección de bacterias pequeñas de ARN

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09:45 min

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January 10, 2012

DOI:

10.3791/3655-v

DOI:

10.3791/3655-v

•

09:45 min

January 10, 2012

•

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Kelly L. Robertson, Gary J. Vora

¹Center for Bio/Molecular Science and Engineering,Naval Research Laboratory

Tags

Locked Nucleic Acid Flow Cytometry Fluorescence In Situ Hybridization FISH Nucleic Acid Sequences Flow-FISH Lysate-based Nucleic Acid Detection Methods Statistical Analysis Variance Analysis Telomere Length Determination Cellular Identification Gene Expression Viral Multiplication Bacterial Community Analysis DNA Oligonucleotide Probes Nucleic Acid Analogs Sensitivity Specificity Melting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes

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Robertson, K. L., Vora, G. J. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection. J. Vis. Exp. (59), e3655, doi:10.3791/3655 (2012).

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