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Alta capacidade método de detecção de vírus Influenza
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February 04, 2012
DOI:
1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Summary
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Este método descreve o uso de sistema de imagem de Infravermelhos corante baseada em para a detecção de A H1N1 no bronchioalveolar o lavado de fluido (BAL) de camundongos infectados em uma alta sensibilidade. Esta metodologia pode ser realizado em um 96 - ou 384-bem prato, requer <volume de 10 uL de material de teste e tem o potencial para a triagem concomitante de agentes patogénicos múltiplos.
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Cite this Article
Kumar, P., Bartoszek, A. E., Moran, T. M., Gorski, J., Bhattacharyya, S., Navidad, J. F., Thakar, M. S., Malarkannan, S. High-throughput Detection Method for Influenza Virus. J. Vis. Exp. (60), e3623, doi:10.3791/3623 (2012).
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