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在体外对HIV - 1核心脱壳
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In vitro Uncoating of HIV-1 Cores
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在体外对HIV - 1核心脱壳

DOI:
10.3791/3384-v

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10:49 min

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November 08, 2011

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1Department of Pathology, Microbiology and Immunology,Vanderbilt University School of Medicine

Capítulos

  • 00:05Título
  • 01:14Production of HIV-1 Particles
  • 03:04Concentration of HIV-1 Virions by Ultracentrifugation
  • 04:30Sucrose Gradient Centrifugation to Isolate Cores
  • 06:36Kinetic Assay of HIV-1 Uncoating
  • 10:21Conclusion

Summary

Tadução automática

Tadução automática

脱壳是在HIV - 1生命周期的早期阶段的重要一步，并定义为衣壳外壳的拆卸和释放病毒核蛋白复合物（vRNP）。在这里，我们展示了隔离HIV - 1病毒颗粒的完整的核心和量化其脱壳技术<em>在体外</em>。

Tags

In Vitro UncoatingHIV-1 CoresRetrovirusesCapsidLipid EnvelopeLentivirusesCA ProteinHexagon LatticePentamersCone StructureViral Ribonucleoprotein ComplexFusionTarget Cell MembraneCytoplasmDisassemblySoluble CAUncoating ProcessIntracellular LocationTimingAmino-acid SubstitutionsStability Of The CapsidHIV-1 InfectionAssaysQuantitative MethodIsolation Of CoresSedimentationConcentrated VirionsDetergent LayerSucrose Gradient

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