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模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞
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September 28, 2011
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Summary
Automatically generated
微RNA（miRNA）是基因表达的重要调节因子，已被证明在许多生物过程中发挥的作用。为了更好地了解miRNA的UTR区的相互作用，我们已经创建了一个全基因组的3 UTR的荧光素酶记者的集合，搭配了一种新的荧光素酶基因的检测试剂，LightSwitch系统。
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LightSwitch Luciferase Assay System3’UTR-reporter ConstructsMicroRNAs (miRNAs)Gene Expression RegulatorsComputational ToolsExpression AssaysProteomics AssaysChromatin-immunoprecipitation-based TechniquesMiRNA-3’UTR InteractionsFunctional EvidenceCell-based SystemGenome-wide Collection Of Human 3’UTR Luciferase ReportersSynthetic MiRNA Target Reporter ConstructsEndogenous 3’UTR Reporter ConstructsStandardized Experimental ProtocolsCo-transfectionMiRNA MimicHigh-throughput Analysis
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Aldred, S. F., Collins, P., Trinklein, N. Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells. J. Vis. Exp. (55), e3343, doi:10.3791/3343 (2011).
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