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Imunologia e Infecção
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El aislamiento de ARN Pseudomonas aeruginosa Que colonizan el tracto gastrointestinal murino
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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El aislamiento de ARN Pseudomonas aeruginosa Que colonizan el tracto gastrointestinal murino

18,827 Views

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08:52 min

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September 28, 2011

DOI:

10.3791/3293-v

DOI:
10.3791/3293-v

•

08:52 min
September 28, 2011

•

6 Views
, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Summary

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Un método fiable para el aislamiento de ARN de<em> Pseudomonas aeruginosa</em> Recuperado de cecums murino se describe. El ARN se recupera de la cantidad y calidad suficientes para qPCR posteriores, perfiles de transcripción, y los experimentos de ARN Seq. Esta técnica se puede adaptar para el aislamiento de ARN de otros microbios intestinales.

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Lopez-Medina, E., Neubauer, M. M., Pier, G. B., Koh, A. Y. RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract. J. Vis. Exp. (55), e3293, doi:10.3791/3293 (2011).

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