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在艾滋病毒感染者病毒载量低于检测限制标准的临床实验放大和量化HIV - 1 RNA
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September 26, 2011
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量化血浆中HIV - 1 RNA水平和测序单一的HIV - 1病毒载量低于检测限（50-75拷贝/ ml）与个人的基因组是困难的。在这里，我们描述了如何提取和量化使用实时PCR检测方法能够可靠措施，HIV - 1 RNA下降到0.3拷贝/ ml，以及如何放大由单一的基因组测序的病毒基因组病毒载量非常低的样品，血浆病毒RNA。
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AmplifyingQuantifyingHIV-1 RNAViral LoadsLimit Of DetectionStandard Clinical AssaysViral GenesInsightViral DynamicsVirus Host InteractionsPatientsNatural ControlCombination Antiretroviral Therapy (cART)Single Genome Sequencing (SGS Protocol)Single-copy Assay (SCA Protocol)Real-time PCR AssaySensitivityVolume Of PlasmaRNA Copy NumberInternal Control TestingDNA ContaminationTriplicate MeasurementsSingle-genome Sequencing Assay (SGS)Non-labor IntensiveLimiting Dilution AssayEndpoint Diluted CDNA Product
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Mens, H., Kearney, M., Wiegand, A., Spindler, J., Maldarelli, F., Mellors, J. W., Coffin, J. M. Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays. J. Vis. Exp. (55), e2960, doi:10.3791/2960 (2011).
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