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Imunologia e Infecção
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Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

DOI:
10.3791/2950-v

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09:20 min

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October 14, 2011

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, ,
1Institute of Plant Sciences,University of Graz, 2Institute for Electron Microscopy and Fine Structure Research,Graz University of Technology

Capítulos

  • 00:05Título
  • 01:38Microwave Assisted Sample Preparation
  • 04:19Trimming and Sectioning
  • 05:36Negative Staining
  • 06:41TEM and Image Analysis
  • 07:52Representative Results of TEM
  • 08:57Conclusion

Summary

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This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Tags

Microwave-assistedRapid DiagnosisPlant Virus DiseasesTransmission Electron MicroscopyUltrastructural ChangesSample PreparationTEM InvestigationsMicrowave FixationEmbeddingBiological SamplesTobacco Mosaic Virus (TMV)Nicotiana Tabacum PlantsUltrastructural AlterationsLeavesCommercially Available Microwave DeviceNegative StainingViral Particles

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