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Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
JoVE Journal
Biologia
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JoVE Journal Biologia
Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

DOI:
10.3791/2852-v

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07:58 min

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August 25, 2011

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, ,
1Department of Biological Sciences,University of Alabama in Huntsville, 2NIDDK-National Institutes of Health

Capítulos

  • 00:05Título
  • 01:12Preparing for Dye Injections
  • 03:15Dye Injections
  • 04:38Prepare the Injected Embryos for Imaging
  • 06:36Time-lapse Image of Embryogenesis
  • 07:29Conclusion

Summary

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This article describes a technique for the visualization of the early events of embryogenesis in the nematode Caenorhabditis elegans.

Tags

Time-lapse MicroscopyEarly EmbryogenesisCaenorhabditis ElegansModel SystemGenetic ResourcesTransformationLaser-based Confocal MicroscopyFluorescently Labeled TagsSpecific Cellular StructuresSpecific ProteinsOrganellesLysosomesMitochondriaFluorescently Labeled DyesMicroinjectionAdult GonadFluorescent Protein TagsHistone ProteinsUbiquitin ProteinsDNA VisualizationCell Cycle Stage IdentificationGFP-tagged UbiquitinUbiquitinated Vesicles DynamicsColocalization AnalysisLysosomal Dye Microinjection

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