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Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
JoVE Journal
Biologia
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JoVE Journal Biologia
Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

DOI:
10.3791/2699-v

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06:15 min

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February 17, 2011

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1Shimadzu,Scientific Instruments

Capítulos

  • 00:05Título
  • 00:54DNA Quantitation
  • 03:27Protein Quantitation
  • 04:37Representative Results of DNA and Protein Quantitation
  • 06:00Conclusion

Summary

Tadução automática

Tadução automática

This communication presents data on the accuracy and reproducibility of the BioSpec-nano UV-VIS spectrophotometer for dsDNA and protein quantitation. Even with ultra-small volumes (1 to 2 L), reproducibility is excellent, while the automated wiping function improves throughput and results in minimal carryover for more precise results.

Tags

Concentration DeterminationNucleic AcidsProteinsMicro-volumeBio-spec Nano SpectrophotometerQuantitation ProceduresMolecular BiologistsSmall-volume AnalysisAccurate ResultsDNA Quantitation MethodsSpecialized OpticsPDA DetectorAutomated FunctionalityMeasurement ReproducibilityMeasurement AccuracySample ContactRapid ScanningThroughputData AnalysisSoftware FeaturesDNA Concentration CalculationSample Dilution FactorNucleic Acid Concentration FactorProtein Concentration Results

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