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에 대한 부정적인 선택 마커의 개발 Entamoeba histolytica</em
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Development of a Negative Selectable Marker for Entamoeba histolytica
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에 대한 부정적인 선택 마커의 개발 Entamoeba histolytica</em

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06:05 min

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December 12, 2010

DOI:

10.3791/2410-v

DOI:
10.3791/2410-v

•

06:05 min
December 12, 2010

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2 Views
, , ,
1Division of Infectious Disease and International Health,University of Virginia Health System

Summary

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우리의 부정적인 선택 시스템의 개발을보고<em> E. histolytica</em> 형질 키메라 단백질의 표현 (FCU1)과 prodrug 5 ​​- fluorocytosine와 선택에 따라. FCU1 단백질은 효모 사이 토신의 deaminase와 phosphoribosyltransferase 유라실의 융합이다. FCU1 표현 증가의 결과<em> E. histolytica</em5 fluorocytosine 향해> 감성.

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Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate

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Abhyankar, M. M., Haviland, S. M., Gilchrist, C. A., Petri, Jr., W. A. Development of a Negative Selectable Marker for Entamoeba histolytica. J. Vis. Exp. (46), e2410, doi:10.3791/2410 (2010).

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