Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Journal

Neurociência

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

DOI:

10.3791/2373-v

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15:40 min

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January 24, 2011

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Christopher Viesselmann*¹, Jason Ballweg*¹, Derek Lumbard*¹, Erik W. Dent

¹Department of Anatomy,University of Wisconsin-Madison

Capítulos

00:00Título
02:23Construction of Imaging Chambers
03:45Cortical Glial Feeder Layer Preparation for Long-term Cultures
07:39Cortical and/or Hippocampal Dissection and Electroporation
12:10Representative Results
14:37Conclusion

Summary

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This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

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Capítulos

Summary

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