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重组逆转录病毒的生产和B细胞的感染

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Recombinant Retroviral Production and Infection of B Cells

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重组逆转录病毒的生产和B细胞的感染

DOI:

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09:19 min

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February 18, 2011

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Celia Keim, Veronika Grinstein, Uttiya Basu²

¹Department of Microbiology and Immunology,Columbia University College of Physicians and Surgeons, ²Herbert Irving Comprehensive Cancer Center,Columbia University

Capítulos

00:05Título
01:24Splenic B-Lymphocyte Isolation and Stimulation
05:42Transfection of Producer Cells
07:26Infection of Stimulated Splenic B cells
08:12Flow Cytometry Analysis
08:40Rescue of AID-Deficiency by Transduction of AID-Deficient B Cells with a Recombinant Retrovirus
08:57Conclusion

Summary

Tadução automática

一个有效率的制度中的一个基因的结构和功能分析<em>体外</em>脾B淋巴细胞的文化描述。这种方法需要利用一个辅助的自由，ecotrophic包装细胞系的重组逆转录病毒的生产。稳定，遗传兴趣在初级淋巴细胞基因的表达，是实现领导在接受类开关重组的B细胞表面抗体的生成。

Tags

Recombinant Retroviral Production B Cells Transgenic Expression Reverse Genetic Approach Heterologous Expression System Phenotype Analysis Gene Over-expression Hematopoetic System Mouse Genetics Human Genes And Diseases Synteny Conservation Gene Disruptions Allelic Replacement Transgenic Mouse Creation Cell Culture Model

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