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Aislamiento y cultivo de células endoteliales pulmonares de ratones recién nacidos
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December 14, 2010
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IsolationCulturePulmonary Endothelial CellsNeonatal MiceResearch ModelVascular BiologyHuman Umbilical Vein Endothelial Cells (HUVECs)Microvascular Endothelial Cells (ECs)AngiogenesisPermeabilityKnockout MiceProtein Function AnalysisIsolation ProtocolCulturing ProtocolMouse Lung Endothelial Cells (MLECs)Collagenase/dispase (C/D) SolutionSingle-cell SuspensionPositive SelectionAnti-PECAM-1 AntibodyDynabeadsMagnetic Particle Concentrator (MPC)
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Sobczak, M., Dargatz, J., Chrzanowska-Wodnicka, M. Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice. J. Vis. Exp. (46), e2316, doi:10.3791/2316 (2010).
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