This content is Free Access.
Isolamento e cultura de células endoteliais pulmonares de camundongos Neonatal
46,462 Views
•
10:46 min
•
December 14, 2010
DOI:
Tags
IsolationCulturePulmonary Endothelial CellsNeonatal MiceResearch ModelVascular BiologyHuman Umbilical Vein Endothelial Cells (HUVECs)Microvascular Endothelial Cells (ECs)AngiogenesisPermeabilityKnockout MiceProtein Function AnalysisIsolation ProtocolCulturing ProtocolMouse Lung Endothelial Cells (MLECs)Collagenase/dispase (C/D) SolutionSingle-cell SuspensionPositive SelectionAnti-PECAM-1 AntibodyDynabeadsMagnetic Particle Concentrator (MPC)
Read Article
Cite this Article
Sobczak, M., Dargatz, J., Chrzanowska-Wodnicka, M. Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice. J. Vis. Exp. (46), e2316, doi:10.3791/2316 (2010).
Copy